圧力制御式送液システム / Pressure-based flow controllers
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圧力制御式送液システム Flow EZ (動画) 制御した圧力をリザーバに加えることで，安定した送液を行う。 |
MPS灌流用自動送液装置 OMIOrgan-on-a-chipでの培養時に，培地や試薬の送液を自動制御する。 |
圧力制御式送液システムとは
制御された空気圧でリザーバ内の試薬を押し出すことで，脈動のない精密な送液を実現します。また，シリンジポンプ等の他の送液方式に比べ，送液の開始や停止指示に対するレスポンスが非常に早いため，マイクロ流体分野の実験に適しています。使用するリザーバサイズに応じて液量を調整できるので，貴重な試薬の少量注入から培地の長時間灌流まで，様々な送液用途に対応可能です。
Fluigentの送液装置
Fluigentはこれまで約20年間，マイクロ流体をはじめとする研究分野や産業向けに，圧力制御式の送液装置を提供してまいりました。世界中の大学，研究機関，企業において数千台規模の導入実績を有しています。
Fluigentの送液装置はいずれも，高精度の圧送方式による安定送液と高速レスポンスを両立しており，微小流量を精密に制御できます。専用ソフトウェアで送液プロトコルを自動化することができ，再現性の高い実験環境を構築することが可能です。
Flow EZ (フロー･イージー)は，液滴生成，細胞培養，マイクロピペット吸引等の幅広いアプリケーションに使用可能な，汎用性の高い送液装置です。装置構成の自由度が高く，シンプルな1液の送液制御から，複数液体の切替えを含む複雑なセットアップまで，実験内容に応じて柔軟に構築できます。
OMI (オミ)は，MPS / Organ-on-a-chipへの送液に特化した自動送液装置です。灌流，再循環，試薬注入等の送液プロトコルを自由に設定することができます。装置ごとインキュベータに入れることができ，操作はタブレットによる遠隔操作で行います。形状やメーカに制限されることなく，幅広いチップへの送液に対応します。
Fluigentでは，これらの送液装置に加え，流量センサやバルブ装置等の多様な関連デバイスも提供しています。
圧力制御式送液装置: デバイスとアプリケーション
カタログ･資料
発表事例 / Publication list
Fluigentの送液システムを用いた成果抜粋
Fluigentの送液システムを用いた成果抜粋
[液滴生成]
Microfluidic Generation of All-Aqueous Double and Triple Emulsions. M Jeyhani et al., small, Vol. 16, Issue 7, 2020, 1906565 (Keywords: Aqueous two-phase systems, Dextran, Double emulsions)
Responsive Janus and Cerberus emulsions via temperature-induced phase separation in aqueous polymer mixtures. M Pavlovic et al., Journal of Colloid and Interface Science Vol. 575, 2020, 88-95 (Keywords: Complex emulsions, Aqueous two-phase systems, Janus particles)
Microfluidic droplet generation based on non-embedded co-flow-focusing using 3D printed nozzle. A Dewandre et al., Sci Rep 10, 21616 (2020) (Keywords: Fluid dynamics, Microfluidics, Droplet generation)
High-Throughput Aqueous Two-Phase System Droplet Generation by Oil-Free Passive Microfluidics. M Mastiani et al., ACS Omega 2018, 3, 8, 9296–9302 (Keywords: Surface tension, Lipids, Liquids)
A microfluidic needle for sampling and delivery of chemical signals by segmented flows. S Feng et al., Appl. Phys. Lett. 111, 183702 (2017) (Keywords: Microfluidic needle, Chemical signals)
pH-Responsive liquid crystal double emulsion droplets prepared using microfluidics. J Y Kwon et al., RSC Adv., 2016,6, 55976-55983 (Keywords: Nematic liquid crystal, Double emulsion)
[液滴生成 (バイオ分野)]
Microfluidics Technology for the Design and Formulation of Nanomedicines. Eman Jaradat et al., Nanomaterials 2021, 11, 3440 (Keywords: Drug delivery, Liposomes, Microfluidics, Nanoparticles, Nanomedicine, PLGA)
Monosized Polymeric Microspheres Designed for Passive Lung Targeting: Biodistribution and Pharmacokinetics after Intravenous Administration. M Agnoletti et al., ACS Nano 2020, 14, 6, 6693–6706 (Keywords: PLGA, Microspheres)
Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens. B Laperrousaz et al., Nucleic Acids Research, 2018, Vol. 46, No. 12 (Keywords: Cell biology, DNA-Mediated Cell Transformation and Nucleic Acids Transfer)
Cell-free extract based optimization of biomolecular circuits with droplet microfluidics. Y Hori et al., Lab Chip, 2017,17, 3037-3042 (Keywords: Biomolecular circuits, Biocircuits, Droplets)
High throughput single cell counting in droplet-based microfluidics. H Lu et al., Scientific Report 7, 2017, 1366 (Keywords: Engineering, Lab-on-a-chip)
[がん関連アプリケーション]
The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination. G Offeddu et al., Communications Biology Vol. 4, 255 (2021) (Keywords: Cancer models, Glycobiology, Metastasis)
Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research. F Duzagac et al., Cancers 2021, 13(4), 737 (Keywords: OOAC, Organoids, Cancer models)
Flow-Induced Transport of Tumor Cells in a Microfluidic Capillary Network: Role of Friction and Repeated Deformation. N Kamyabi et al., Cel. Mol. Bioeng. (2017) 10: 563 (Keywords: Tumor cells, Microfluidics, Capillary)
FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells. K Perez-Toralla et al., Methods in molecular biology (Clifton, N.J.): 211-220 (Keywords: FISH, Gene amplification, Microfluidic)
[血液関連アプリケーション]
A Microfluidic Model of Hemostasis Sensitive to Platelet Function and Coagulation. R M Schoeman et al., Cel. Mol. Bioeng. (2017) 10: 3 (Keywords: Biorheology, Biotransport, Platelet)
Direct Tracking of Particles and Quantification of Margination in Blood Flow. E J Carboni et al., Biophys. Journal, Vol 111, 7, 1487-1495 (2016) (Keywords: Margination, Drug delivery, Blood flow)
[セルソーティング]
Cell Sorting Using Electrokinetic Deterministic Lateral Displacement. B Ho et al., Micromachines 2021, 12(1), 30 (Keywords: Cell sorting, DLD, Electrokinetics)
A 3D hydrodynamic flow-focusing device for cell sorting. X Yuan et al., , Microfluidics and Nanofluidics Vol. 25, 23 (2021) (Keywords: 3D flow-focusing, Multilayer structures,
Cell sorting)
[ハイスループットスクリーニング]
Crossed flow microfluidics for high throughput screening of bioactive chemical–cell interactions. Z Tong et al., Lab Chip, 2017, 17, 501-510 (Keywords: High throughput screening, Selective cell capture, Crossed laminar flow)
[マイクロピペット吸引法]
Micropipette aspiration: A unique tool for exploring cell and tissue mechanics
in vivo. K Guevorkian et al., Methods in cell biology 139 (Keywords: Actomyosin contractility, Cell adhesion, Cell and tissue mechanics)
[Flow EZ / Push-Pullを使用した2021年以降の発表]
Fully 3D-printed soft robots with integrated fluidic circuitry. JD Hubbard et al.
The effects of luminal and trans-endothelial fluid flows on the extravasation and tissue invasion of tumor cells in a 3D in vitro microvascular platform. C Hajal et al.
CloneSeq: A highly sensitive analysis platform for the characterization of 3D-cultured single-cell-derived clones. D Bavli et al.
A microdevice platform for characterizing the effect of mechanical strain magnitudes on the maturation of iPSC-Cardiomyocytes. W Dou et al.
A core-annular liquid–liquid microextractor for continuous processing. ZX Yu et al.
A 3D Printed Morphing Nozzle to Control Fiber Orientation during Composite Additive Manufacturing. CD Armstrong et al.
Microfluidic electrical impedance assessment of red blood cell-mediated microvascular occlusion. S Tyagi et al.
Optimizing pressure-driven pulsatile flows in microfluidic devices. SM Recktenwald et al.
Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip. N Compera et al.
Time-resolved investigation of mesoporous silica microsphere formation using in situ heating optical microscopy. AJ Fijneman et al.
Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting. L Descamps et al.
Deformation and rupture of microcapsules flowing through constricted capillary. D Diamond et al.
Multiple objects interacting with a solidification front. Z Han et al.
Heart Muscle Microphysiological System for Cardiac Liability Prediction of Repurposed COVID-19 Therapeutics. B Charrez et al.
Porous Silicon Biosensor for the Detection of Bacteria through Their Lysate. R Vercauteren et al.
Highly parallelized human embryonic stem cell differentiation to cardiac mesoderm in nanoliter chambers on a microfluidic chip. AR Vollertsen et al.
Microheart: A microfluidic pump for functional vascular culture in microphysiological systems. GS Offeddu et al.
Producing Hollow Polymer Microneedles Using Laser Ablated Molds in an Injection Molding Process. T Evens et al.
Growth response and recovery of Corynebacterium glutamicum colonies on single-cell level upon defined pH stress pulses. K El et al.
Acoustofluidic Medium Exchange for Preparation of Electrocompetent Bacteria Using Channel Wall Trapping. M Gerlt et al.
The Functional Nanopore (FuN) Screen: A Versatile Genetic Assay to Study and Engineer Protein Nanopores in Escherichia coli. W Weber et al.
Synthetic cell-based materials extract positional information from morphogen gradients Supplementary Information. A Dupin et al.
The Use of Micropipette Aspiration to Measure Cortex Tension in HeLa Cells and Cardiac Myocytes. S Baillargeon et al.
